Thalassemia is an important hematological disorder. The possibility of iron overload development may be increase by Interaction between thalassemia and HFE gene mutations. This study aim to investigate the possible association between serum hepcidin level as indicator of iron concentration and the presence of HFE gene mutations. Excess iron overload increases the risk of liver cirrhosis,cancer,hypogonadism,arthritis, cardiac arrhythmia, heart failure, retinal degeneration, diabetes mellitus, neurodegenerative diseases (Alzheimer's,Parkinson's, Huntington's), and premature death.