This
second edition details new emerging areas of zebrafish research focusing on genetics
and genomics, techniques for developing and analyzing zebrafish disease models,
and methods for neuroscience. Zebrafish:
Methods and Protocols, Second Edition guides readers through methods for mutagenesis
and genome editing in zebrafish,  applications
of GFP-expressing transgenic fish, techniques for cancer models, imaging of infection
and host-pathogen interactions, metabolism and transport of lipids, and  the structure and function of neural circuits
and their role in generating behavior. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.



 



 



Authoritative and practical Zebrafish: Methods and Protocols, Second Edition is a useful
complement to the first book for new and experienced zebrafish researcher
alike.