This book constitutes the refereed proceedings of the First International Workshop on Collaborative Intelligence and Autonomy in Image-Guided Surgery, COLAS 2025, held in Conjunction with MICCAI 2025, in Daejeon, South Korea, on September 23, 2025.

The volume includes 19 papers which were carefully reviewed and selected from 31 submissions. The COLAS 2025 workshop brought together researchers, surgeons, and industry leaders to discuss the latest innovations and applications of the new technologies in enhancing surgical precision, skill learning, decision-making, and patient outcomes for surgery.