This book constitutes the refereed proceedings of the 9th International Conference on Typed Lambda Calculi and Applications, TLCA 2009, held in Brasilia, Brazil in July 2008 in conjunction with RTA 2007, the 19th International Conference on Rewriting Techniques and Applications as part of RDP 2009, the 5th International Conference on Rewriting, Deduction, and Programming. The 27 revised full papers presented together with 2 invited talks were carefully reviewed and selected from 53 submissions. The papers present original research results that are broadly relevant to the theory and applications of typed calculi and address a wide variety of topics such as proof-theory, semantics, implementation, types, and programming.