Now available as a three-volume set, the second edition of this popular handbook provides expanded coverage of many important topics and recent developments in computational biology. With updates in each chapter, the book incorporates the latest research and applications in systems biology, including next-generation sequencing. This volume explores the tools and techniques for large-scale pyrosequencing, massively parallel sequencing, single molecule real-time sequencing, short read error correction, referencing genomes, high-throughput sequencing and genome assembly, metagenomics, and plant genome annotation.