The second edition of the highly acclaimed Differential Display Methods and Protocols consists of a wider-ranging collection of chapters that highlight both recent methodological refinements and some fine examples of differential display (DD) applications in recent years. Part I covers gene discovery methodologies that have a unique place in the modern molecular biological toolbox for gene expression analysis while Part II demonstrates the power of DD technology by showcasing select applications that have led to the discovery of many important genes involved in viral infection, Prion disease, cancer, ovulation, circadian clock, floral color, transcription re3pression, gene silencing, mRNA polymorphism and protein-RNA interaction.